Root Colonization of Root and Nodule Endophytic Bacteria in Legume and Non Legume Plants Grown in Liquid Medium
نویسندگان
چکیده
Endophytes have a symbiotic relationship with the different parts of plants and could play a very important role in supporting the plant growth. In present study, 11 most efficient isolates were selected out of more than 200 endophytic bacteria isolated previously from roots of chickpea (Cicer arietinum), field pea (Pisum sativum), Lucerne (Medicago sativa), wheat (Triticum aestivum) and oat (Avena sativa) and nodules of chickpea and field pea. To know their extent of establishment in different host and nonhost tissues root exudates of four hosts’ chickpea, field pea, wheat and oat were collected at 7 and 14 days growth. Root exudates of chickpea, field pea, wheat and oat attracted different endophytic bacteria to different extent. Isolate ORE27 exhibited maximum chemotactic ratio towards root exudates of all crops followed by isolate WRE4 towards oat, wheat and chickpea root exudates. Presence of root exudates, promoted the growth of different endophytes in MS medium tubes. In field pea roots maximum endophytic colonization after 21 days was observed followed by chickpea, oat and wheat. All the 11 endophytic bacteria entered in pea roots, 10 in chickpea, 8 in oat and wheat roots in MS medium tubes. No host specificity among 11 endophytic bacteria and four hosts could be observed at any of the stage of root colonization. All the endophytic bacteria are being identified
منابع مشابه
Isolation and Characterization of Nodule Associated Bacteria from Chickpea and their Potential for Plant Growth Promotion
Chickpea (Cicer arietinum) is an important legume crop grown under rainfed agriculture in India. It’s economic success relies on symbiotic nitrogen fixation (SNF) with root nodulating bacterium Mesorhizobium ciceri. These bacteria regularly interact with other rhizospheric microorganisms as well as host endophytic microorganisms. The endophytic bacteria reside latently or colonize the plant tis...
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